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2009-06-15

The cells were then incubated with the primary antibody specific for γ-H2AX (Cell Signaling Technologies) at a 1:100 dilution in the DELFIA assay buffer (PerkinElmer) for 2 h at room temperature Gamma H2AX (γH2AX) is the phosphorylated version of histone H2AX and is a marker for double-stranded breaks (DSBs) caused by DNA damage. As a result of DSBs, H2AX becomes phosphorylated at serine-139 (p Ser139). cell death and tumorigenesis, so they are of keen interest in understanding cancerous mechanisms as well as developing therapeutic treatments. One of the most characterized early markers of DNA DSBs is the phosphorylation of the histone 2AX (H2AX) to γH2AX. Here, a positive feedback loop is created between γH2AX and phosphatidyl inositol 3' H2AX is a member of the histone protein H2A family; the other two members are H2A1-H2A2 and H2AZ .

Gamma h2ax cell signaling

Share by email Anti-gamma H2A.X (phospho S139) antibody Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

Listed are ELISA Kits for the detection of H2AX, an alias name of H2A histone family member X. The human protein, encoded by the gene H2AFX, is 143 amino acid residues long and has a mass of 15,145 daltons. Anti Gamma H2ax, supplied by Cell Signaling Technology Inc, used in various techniques.

DNA double-strand breaks (DSBs) are extremely dangerous lesions with severe consequences for cell survival and the maintenance of genomic stability. In higher eukaryotic cells, DSBs in chromatin promptly initiate the phosphorylation of the histone H2A variant, H2AX, at Serine 139 to generate gamma-H2AX.

Supplier also known as. CST, Cell … 2016-03-31 The {gamma}H2AX Assay for Genotoxic and Nongenotoxic Agents: Comparison of H2AX Phosphorylation with Cell Death Response DNA double-strand breaks (DSBs) and blocked replication forks resulting from bulky adducts and inhibitors of replication activate the DNA damage response (DDR), a signaling pathway marked by phosphorylation of histone 2AX (H2AX). gamma-h2ax-phospho-s139-antibody-c-terminal-ab228655.pdf.

2013-11-01

gamma-h2ax-phospho-s139-antibody-c-terminal-ab228655.pdf. Epigenetics and Nuclear Signaling Histones Variants. Share by email Anti-gamma H2A.X (phospho S139) antibody Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Variant histone H2A which replaces conventional H2A in a subset of nucleosomes.

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DNA damage, caused by ionizing radiation, UV-light, or radiomimetic agents, results in rapid phosphorylation of H2A.X at Ser139 by PI3K-like kinases, including ATM, ATR, and DNA-PK (2,3). Phosphorylated H2AX (gamma-H2AX) is essential to the efficient recognition and (or) repair of DNA double strand breaks (DSBs), and many molecules, often thousands, of H2AX become rapidly phosphorylated at the site of each nascent DSB. An antibody to gamma-H2AX reveals that this highly amplified process generates nuclear foci. The gamma-H2AX assay was capable of detecting DNA damage at levels 100-fold below the detection limit of the alkaline comet assay. The gamma-H2AX signal was quantitative with a linear increase of the gamma-H2AX signal over two orders of magnitude.

P16104, Q4ZGJ7, Q6IAS5. Gene Synonyms. H2AX, H2a.x, H2afx, H2A/X.
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Feb 6, 2007 Interindividual difference in the gamma-H2AX signal in response to ionizing radiation and the DSB-inducing drug calicheamicin was almost 2-fold

Rabbit Polyclonal Anti-gamma H2AX [p Ser139] Antibody DNA Double-strand break marker cited in 26 publications. Validated: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P. Tested Reactivity: Human, Mouse, Canine, and more.

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H2AXΔ/Δ mice are smaller, sensitive to ionizing radiation, defective in class switch recombination and spermatogenesis while cells from the mice demonstrate

γ-H2AX is a specific and efficient coordinator of DDR signaling.